In this talk, I will present our laboratory’s recent research efforts in rapid continuous projection 3D bioprinting to create 3D scaffolds and tissue constructs using a variety of biomaterials. These 3D biomaterials are functionalized with precise control of micro-architecture, mechanical (e.g. stiffness and Poisson’s ratio), chemical, and biological properties . Design, fabrication, and experimental results will be discussed. Such functional biomaterials allow us to investigate cell-microenvironment interactions at nano- and micro-scales in response to integrated physical and chemical stimuli. From these fundamental studies we can create both in vitro and in vivo microphysiological systems such as a human liver tissue for tissue regeneration, disease modeling, and drug discovery . To vascularize these engineered tissues, we have also developed a prevascularization strategy by using the rapid 3D bioprinting method . Multiple cell types mimicking the native vascular cell composition were encapsulated directly into hydrogels with precisely controlled distribution.