This presentation highlights results from our biomaterials and molecular based approaches to direct the immune system toward tolerance and suppression. Microparticle-based systems have been designed as vaccines to retrain the immune system to correct aberrant activation toward self-antigens. Using a microparticle formulation consisting of a combination of suppressive factors we have demonstrated prevention and reversal of type 1 diabetes in non-obese diabetic mice, as well as treatment of multiple sclerosis in an EAE mouse model. On the molecular scale, we have also developed tissue-anchored enzymes – chimeric enzyme-carbohydrate binding fusion proteins in order to direct localized immunometabolism toward suppression. Specifically, indoleamine 2,3 dioxygenase, which depletes tryptophan through the kynurenine pathway, is fused to galectin 3, which binds extracellular glycans and affords prolonged tissue retention. This approach has provided potent, confined metabolic programming in inflammation models including osteoarthritis and periodontal disease.
Benjamin G. Keselowsky is a Professor in the J. Crayton Pruitt Family Department of Biomedical Engineering at the University of Florida. He received his BS, Chemical Engineering from the University of South Florida, PhD, Bioengineering from Georgia Institute of Technology and joined as faculty at the University of Florida in 2005. He is currently funded by the NIH (R01DK098589, R01DE027301, R01AI133623, T32 DK108736), with past funding the National Science Foundation, the Arthritis Foundation, the Juvenile Diabetes Research Foundation and Pfizer. He serves on editorial boards for the Journal of Biomedical Materials Research-Part A, and the Journal of Materials Chemistry B, Scientific Reports, the Journal of Immunology and Regenerative Medicine, ACS Biomaterials Science and Engineering, and is an Associate Editor for Nanomedicine: Nanotechnology, Biology and Medicine. He is a standing member on the NIH Biomaterials and Biointerfaces Study Section. He is a recipient of the 2015 University of Florida Research Foundation Professorship Award, the 2016 UF Technology Innovator Award, the 2017 UF Preeminence Term Professorship Award and was inducted as a Fellow of the American Institute for Medical and Biological Engineering (AIMBE) in 2018.